The Tottenham striker had an injury scare against Dinamo Zagreb in the Europa League on Thursday when he was substituted late on after a heavy challenge.
Kane had an ice pack on his right knee on the bench after coming off but he is fit to start at Emirates Stadium in a huge boost for Spurs.
Kane leads the lead against Arsenal and starts alongside Gareth Bale and Heung-min Son.
Lucas Moura starts in the No10 position having impressed in the recent wins over Burnley and Crystal Palace.
Tanguy Ndombele, who was also substituted on Thursday following some rough treatment from Dinamo Zagreb, is fit and starts in midfield alongside Pierre-Emile Hojbjerg.
Matt Doherty gets the nod ahead of Serge Aurier at right-back, while Toby Alderweireld and Sergio Reguilon return to the back four.
Tottenham line-up: Lloris; Doherty, Sanchez, Alderweireld, Reguilon; Hojbjerg, Ndombele; Bale, Lucas, Son; Kane.
Subs: Hart, Aurier, Dier, Davies, Sissoko, Winks, Dele, Lamela, Vinicius.
Venue: Emirates Stadium, Sunday March 14, 16.30